|Title||In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System.|
|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||X Zhang, NO Abutaleb, E Salmon, and GA Truskey|
|Journal||Methods in Molecular Biology (Clifton, N.J.)|
|Pagination||77 - 90|
Human tissue-engineered blood vessels (TEBVs) that exhibit vasoactivity can be used to test drug toxicity, modulate pro-inflammatory cytokines, and model disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialized TEBVs in situ with smaller volumes and higher throughput than previously reported. Both primary and induced pluripotent stem cell (iPSC)-derived cells can be used. Four collagen TEBVs with 600μm inner diameter and 2.9 mm outer diameter are fabricated by pipetting a solution of collagen and medial cells into a three-layer acrylic mold. After gelation, the TEBVs are released from the mold and dehydrated. After suturing the TEBVs in place and changing the mold parts to form a perfusion chamber, the TEBVs are endothelialized in situ, and then media is perfused through the lumen. By removing 90% of the water after gelation, the TEBVs become mechanically strong enough for perfusion at the physiological shear stress of 0.4 Pa within 24 h of fabrication and maintain function for at least 5 weeks.
|Short Title||Methods in Molecular Biology (Clifton, N.J.)|