Title | Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture. |
Publication Type | Journal Article |
Year of Publication | 1990 |
Authors | GA Truskey, DP Nicolakis, D DiMasi, A Haberman, and RW Swartz |
Journal | Biotechnology and Bioengineering |
Volume | 36 |
Issue | 8 |
Start Page | 797 |
Pagination | 797 - 807 |
Date Published | 10/1990 |
Abstract | The growth of two lymphocyte cell lines, a hybridoma cell line and a human cutaneous T cell lymphoma (HuT78), was studied in fed-batch culture, and unstructured models of growth developed. A criteria was established to insure that the growth rate varied by less than a specified tolerance throughout the culture period. Glutamine and serum were growth-limiting nutrients for both cell lines with half-maximal growth rates at 0. 53 mM glutamine and 0. 55%(v/v) serum for the hybridoma cells and 0. 21 mM glutamine and 1. 5% serum for the HuT-78 cells. Over the range of glucose concentrations from 5. 5 mM to 28 mM, the specific growth rate of hybridoma cells was independent of glucose concentration, whereas glucose concentrations above 5. 5 mM inhibited HuT-78 growth. For both cell lines, the growth rate was significantly inhibited by the addition of ammonium, although the hybridoma cell line was more affected by ammonia than was the HuT-78 cell line. Growth of HuT-78 cells increased in the presence of interleukin-2. Unstructured models for the hybridoma cells were similar to other models presented in the literature. Applications of these models to adoptive immunotherapy are discussed. |
DOI | 10.1002/bit.260360807 |
Short Title | Biotechnology and Bioengineering |