Title | Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion. |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | VD Bhat, GA Truskey, and WM Reichert |
Journal | Journal of Biomedical Materials Research |
Volume | 41 |
Issue | 3 |
Start Page | 377 |
Pagination | 377 - 385 |
Date Published | 09/1998 |
Abstract | A preadsorbed layer of "heterogeneous" integrin-dependent and -independent protein was used to enhance initial integrin-mediated endothelial cell attachment and spreading. Glass substrates were treated with fibronectin (Fn) and avidin coupled through adsorbed biotinylated bovine serum albumin (b-BSA). The slides then were seeded with biotinylated BAEC. Control "homogeneous" surfaces were slides adsorbed with either Fn or avidin coupled to b-BSA. The cells were incubated for 0.5 h in serum-containing media and exposed to a range of shear stresses in a laminar flow variable-height flow chamber. The critical shear stress to detach 50% of the seeded cells on the heterogeneous ligand surface was significantly greater than for either of the control homogeneous ligand systems (p < 0.001). Cellular spreading during the initial period of 0-2 h also was higher (p < 0.05) on the heterogeneous ligand-treated surface than on the surface of either of the homogeneous controls. The close contact area of the cell membrane with the substrate 1 h after seeding in serum-containing media was measured using TIRFM. Cells attached onto the heterogeneous ligand-treated surfaces had a significantly (p < 0.01) higher area of close contact with the substrate, which is consistent with a greater degree of attachment and spreading. The results indicate that the combination of integrin-dependent and -independent adhesion systems using heterogeneous ligands further enhances initial endothelial cell attachment and spreading. |
DOI | 10.1002/(sici)1097-4636(19980905)41:3<377::aid-jbm6>3.0.co;2-9 |
Short Title | Journal of Biomedical Materials Research |