Effect of type I interferon on engineered pediatric skeletal muscle: a promising model for juvenile dermatomyositis.

TitleEffect of type I interferon on engineered pediatric skeletal muscle: a promising model for juvenile dermatomyositis.
Publication TypeJournal Article
Year of Publication2024
AuthorsLT Covert, H Patel, A Osman, L Duncan, J Dvergsten, and GA Truskey
JournalRheumatology (Oxford, England)
Volume63
Issue1
Start Page209
Pagination209 - 217
Date Published01/2024
Abstract

<h4>Objective</h4>To investigate pathogenic mechanisms underlying JDM, we defined the effect of type I IFN, IFN-α and IFN-β, on pediatric skeletal muscle function and expression of myositis-related proteins using an in vitro engineered human skeletal muscle model (myobundle).<h4>Methods</h4>Primary myoblasts were isolated from three healthy pediatric donors and used to create myobundles that mimic functioning skeletal muscle in structural architecture and physiologic function. Myobundles were exposed to 0, 5, 10 or 20 ng/ml IFN-α or IFN-β for 7 days and then functionally tested under electrical stimulation and analyzed immunohistochemically for structural and myositis-related proteins. Additionally, IFN-β-exposed myobundles were treated with Janus kinase inhibitors (JAKis) tofacitinib and baricitinib. These myobundles were also analyzed for contractile force and immunohistochemistry.<h4>Results</h4>IFN-β, but not IFN-α, was associated with decreased contractile tetanus force and slowed twitch kinetics. These effects were reversed by tofacitinib and baricitinib. Type I IFN paradoxically reduced myobundle fatigue, which did not reverse after JAKi. Additionally, type I IFN correlated with MHC I upregulation, which normalized after JAKi treatment, but expression of myositis-specific autoantigens Mi-2, melanocyte differentiation-associated protein 5 and the endoplasmic reticulum stress marker GRP78 were variable and donor specific after type I IFN exposure.<h4>Conclusion</h4>IFN-α and IFN-β have distinct effects on pediatric skeletal muscle and these effects can partially be reversed by JAKi treatment. This is the first study illustrating effective use of a three-dimensional human skeletal muscle model to investigate JDM pathogenesis and test novel therapeutics.

DOI10.1093/rheumatology/kead186
Short TitleRheumatology (Oxford, England)