|Title||Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||NO Abutaleb, and GA Truskey|
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
|Short Title||Star Protocols|