Title | Basal cell adhesion molecule/lutheran protein. The receptor critical for sickle cell adhesion to laminin. |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | M Udani, Q Zen, M Cottman, N Leonard, S Jefferson, C Daymont, G Truskey, and MJ Telen |
Journal | The Journal of Clinical Investigation |
Volume | 101 |
Issue | 11 |
Start Page | 2550 |
Pagination | 2550 - 2558 |
Date Published | 06/1998 |
Abstract | Sickle red cells bind significant amounts of soluble laminin, whereas normal red cells do not. Solid phase assays demonstrate that B-CAM/LU binds laminin on intact sickle red cells and that red cell B-CAM/LU binds immobilized laminin, whereas another putative laminin binding protein, CD44, does not. Ligand blots also identify B-CAM/LU as the only erythrocyte membrane protein(s) that binds laminin. Finally, transfection of murine erythroleukemia cells with human B-CAM cDNA induces binding of both soluble and immobilized laminin. Thus, B-CAM/LU appears to be the major laminin-binding protein of sickle red cells. Previously reported overexpression of B-CAM/LU by epithelial cancer cells suggests that this protein may also serve as a laminin receptor in malignant tumors. |
DOI | 10.1172/JCI1204 |
Short Title | The Journal of Clinical Investigation |