Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow

TitleRole of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow
Publication TypeJournal Article
Year of Publication1991
AuthorsIuliano, DJ, Proulx, TL, Saavedra, SS, and Truskey, GA
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume21
Start Page23
Pagination23 - 25
Date Published01/1991
Abstract

Forces exerted on adherent calls by fluid shear stresses can cause cell detachment. In this study, we examined the effect of cell spreading, actin fiber density, and the conformation of adsorbed adhesion proteins in promoting cell adhesion after a brief exposure to shear stresses between 5-80 dyne/cm2 in a parallel plate flow channel. Cell spreading led to increased adhesion. Cell spreading and adhesion were greater when the surface contained preadsorbed fibronectin. Spreading was accompanied by formation of cytoskeleton which strengthened the adhesive contact between the cell and surface. Similar numbers of cells were detached from hydrophobic and hydrophilic glass containing preadsorbed in even though the cell binding domain was in different conformations.

Short TitleAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED