Point-of-Care Rapid Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.

TitlePoint-of-Care Rapid Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.
Publication TypeJournal Article
Year of Publication2016
AuthorsNoviani, M, Jamiolkowski, RM, Grenet, JE, Lin, Q, Carlon, TA, Qi, L, Jantzen, AE, Milano, CA, Truskey, GA, and Achneck, HE
JournalASAIO Journal
Date Published01/2016
Abstract

The most promising alternatives to heart transplantation are left ventricular assist devices and artificial hearts; however their use has been limited by thrombotic complications. To reduce these, sintered titanium (Ti) surfaces were developed, but thrombosis still occurs in ~7.5% of patients. We have invented a rapid-seeding technology to minimize the risk of thrombosis by rapid endothelialization of sintered Ti with human cord blood-derived endothelial cells (hCB-ECs). hCB-ECs were seeded within minutes onto sintered Ti and exposed to thrombosis-prone low fluid flow shear stresses. The hCB-ECs adhered and formed a confluent endothelial monolayer on sintered Ti. The exposure of sintered Ti to 4.4 dynes/cm for 20 hr immediately following rapid-seeding resulted in ~70% cell adherence. The cell adherence was not significantly increased by additional ex vivo static culture of rapid-seeded sintered Ti prior to flow exposure. In addition, adherent hCB-ECs remained functional on sintered Ti, as indicated by flow-induced increase in nitric oxide secretion and reduction in platelet adhesion. After 15-day ex vivo static culture, the adherent hCB-ECs remained metabolically active, expressed EC functional marker thrombomodulin, and reduced platelet adhesion. In conclusion, our results demonstrate the feasibility of rapid-seeding sintered Ti with blood-derived hCB-ECs to generate a living antithrombotic surface.

Short TitleASAIO Journal