|Title||Point-of-Care Rapid Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Noviani, M, Jamiolkowski, RM, Grenet, JE, Lin, Q, Carlon, TA, Qi, L, Jantzen, AE, Milano, CA, Truskey, GA, and Achneck, HE|
The most promising alternatives to heart transplantation are left ventricular assist devices and artificial hearts; however their use has been limited by thrombotic complications. To reduce these, sintered titanium (Ti) surfaces were developed, but thrombosis still occurs in ~7.5% of patients. We have invented a rapid-seeding technology to minimize the risk of thrombosis by rapid endothelialization of sintered Ti with human cord blood-derived endothelial cells (hCB-ECs). hCB-ECs were seeded within minutes onto sintered Ti and exposed to thrombosis-prone low fluid flow shear stresses. The hCB-ECs adhered and formed a confluent endothelial monolayer on sintered Ti. The exposure of sintered Ti to 4.4 dynes/cm for 20 hr immediately following rapid-seeding resulted in ~70% cell adherence. The cell adherence was not significantly increased by additional ex vivo static culture of rapid-seeded sintered Ti prior to flow exposure. In addition, adherent hCB-ECs remained functional on sintered Ti, as indicated by flow-induced increase in nitric oxide secretion and reduction in platelet adhesion. After 15-day ex vivo static culture, the adherent hCB-ECs remained metabolically active, expressed EC functional marker thrombomodulin, and reduced platelet adhesion. In conclusion, our results demonstrate the feasibility of rapid-seeding sintered Ti with blood-derived hCB-ECs to generate a living antithrombotic surface.
|Short Title||ASAIO Journal|