|Title||Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Jamiolkowski, RM, Kang, SD, Rodriguez, AK, Haseltine, JM, Galinat, LJ, Jantzen, AE, Carlon, TA, Darrabie, MD, Arciniegas, AJ, Mantilla, JG, Haley, NR, Noviani, M, Allen, JD, Stabler, TV, Frederiksen, JW, Alzate, O, Keil, LG, Liu, S, Lin, F-H, Truskey, GA, and Achneck, HE|
|Pagination||447 - 460|
Peripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor type 4 antagonist AMD3100, as well as via a diluted whole blood incubation (DWBI).Porcine pBD-ECs were isolated using AMD3100 and DWBI and tested for EC markers, acetylated LDL uptake, growth kinetics, metabolic activity, flow-mediated nitric oxide production and seeded onto titanium tubes implanted into vessels of pigs.DWBI increased the yield of porcine pBD-ECs 6.6-fold, and AMD3100 increased the yield 4.5-fold. AMD3100-mobilized ECs were phenotypically indistinguishable from nonmobilized ECs. In porcine implants, the cells expressed endothelial nitric oxide synthase, reduced thrombin-antithrombin complex systemically and prevented thrombosis.Administration of AMD3100 and the DWBI method both increase pBD-EC yield.
|Short Title||Regenerative medicine|