Human umbilical cord blood-derived endothelial cells reendothelialize vein grafts and prevent thrombosis.

TitleHuman umbilical cord blood-derived endothelial cells reendothelialize vein grafts and prevent thrombosis.
Publication TypeJournal Article
Year of Publication2010
AuthorsBrown, MA, Zhang, L, Levering, VW, Wu, J-H, Satterwhite, LL, Brian, L, Freedman, NJ, and Truskey, GA
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume30
Issue11
Start Page2150
Pagination2150 - 2155
Date Published11/2010
Abstract

OBJECTIVE: To accelerate vein graft reendothelialization and reduce vein graft thrombosis by infusing human umbilical cord blood-derived endothelial cells (hCB-ECs) because loss of endothelium contributes to vein graft thrombosis and neointimal hyperplasia. METHODS AND RESULTS: Under steady flow conditions in vitro, hCB-ECs adhered to smooth muscle cells 2.5 to 13 times more than ECs derived from peripheral blood or human aorta (P<0.05). Compared with peripheral blood and human aorta ECs, hCB-ECs had 1.4-fold more cell surface α(5)β(1) integrin heterodimers per cell (P<0.05) and proliferated on fibronectin 4- to 10-fold more rapidly (P<0.05). Therefore, we used hCB-ECs to enhance reendothelialization of carotid interposition vein grafts implanted in NOD.CB17-Prkdc(scid)/J mice. Two weeks postoperatively, vein grafts from hCB-EC-treated mice demonstrated approximately 55% reendothelialization and no luminal thrombosis. In contrast, vein grafts from sham-treated mice demonstrated luminal thrombosis in 75% of specimens (P<0.05) and only approximately 14% reendothelialization. In vein grafts from hCB-EC-treated mice, 33±10% of the endothelium was of human origin, as judged by human major histocompatibility class I expression. CONCLUSIONS: The hCB-ECs adhere to smooth muscle cells under flow conditions in vitro, accelerate vein graft reendothelialization in vivo, and prevent vein graft thrombosis. Thus, hCB-ECs offer novel therapeutic possibilities for vein graft disease.

DOI10.1161/ATVBAHA.110.207076
Short TitleArteriosclerosis, Thrombosis, and Vascular Biology