Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.

TitleEndothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose.
Publication TypeJournal Article
Year of Publication2013
AuthorsKinev, AV, Levering, V, Young, K, Ali-Osman, F, Truskey, GA, Dewhirst, MW, and Il'yasova, D
JournalCancer Investigation (Informa)
Volume31
Issue5
Start Page359
Pagination359 - 364
Date Published06/2013
Abstract

Identification of measurable nontransient responses to low-dose radiation in human primary cell cultures remains a problem. To this end, circulating endothelial colony-forming (progenitor) cells (ECFCs) were examined as an experimental model. ECFCs were isolated from three cord blood donors. Cells were positive for endothelial cell markers and remained highly proliferative after long-term cryopreservation. A single dose of X-ray radiation (0.06-0.38 Gy) inhibited ECFC culture growth. This effect was evident at 48 hours and persisted up to 72 hr postirradiation. Such protracted cytostatic response of ECFCs to low-dose radiation suggests that ECFC primary cultures can be used to study low-dose radiation effects.

DOI10.3109/07357907.2013.789903
Short TitleCancer Investigation (Informa)