Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers

TitleEffect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers
Publication TypeJournal Article
Year of Publication1996
AuthorsBurmeister, JS, Vrany, JD, Reichert, WM, and Truskey, GA
JournalJournal of Biomedical Materials Research
Volume30
Issue1
Start Page13
Pagination13 - 22
Date Published01/1996
Abstract

The effect of substrate surface hydrophobicity on fibronectin (Fn) adsorption and endothelial cell adhesion strength was studied. Bovine aortic endothelial cells (BAEC) were plated for 2 h with and without preadsorbed Fn on slides coated with homopolymers and copolymers of hydrophilic polyhydroxyethylmethacrylate (polyHEMA) and hydrophobic polyethylmethacrylate (polyEMA). The amount of adsorbed Fn was determined using 125I-labeled Fn. BAEC were cultured in serum-free medium for 2 h and subjected to a brief exposure of laminar flow in a variable-height flow chamber that provided a range of shear stresses of 15-185 dynes/cm2. The critical shear stress to detach 50% of the cells increased with increasing EMA content to a maximum at 20% HEMA/80% EMA copolymer irrespective of the presence of preadsorbed Fn. However, the critical force increased even though there were similar amounts of Fn adsorbed on all substrates. Thus, the force to detach cells did not increase solely with increasing amounts of adsorbed Fn.

DOI10.1002/(SICI)1097-4636(199601)30:13.0.CO;2-U
Short TitleJournal of Biomedical Materials Research