Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy

TitleDynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy
Publication TypeJournal Article
Year of Publication1995
AuthorsOlivier, LA, Reichert, WM, and Truskey, GA
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume29
Start Page565
Pagination565 - 566
Date Published01/1995
Abstract

To study the effect of luminal fluid stress on the abluminal cell surface and its adhesion sites, called focal contacts, total internal fluorescence reflectance microscopy is developed for measuring the dynamic response of endothelial cell focal contacts to flow in vitro. This method is used to illuminate the lower cell membrane so that focal contact movement can be followed over time. Based on the results, it is indicated that abluminal cell contour modulation occurs at a moderate level under static and low shear conditions, increasing substantially at higher shear rates. Moreover, at early flow time, the cell contour is moving away from the surface and focal contact sites are decreasing in response to the fluid stress.

Short TitleAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED