Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.

TitleDifferentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.
Publication TypeJournal Article
Year of Publication2021
AuthorsNO Abutaleb, and GA Truskey
JournalStar Protocols
Volume2
Start Page100394
Issue2
Date Published06/2021
Abstract

Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).

DOI10.1016/j.xpro.2021.100394
Short TitleStar Protocols