Altered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers.

TitleAltered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers.
Publication TypeJournal Article
Year of Publication1992
AuthorsLaney, TV du, Cherry, RS, Coppinger, JA, and Truskey, GA
JournalBiotechnology Progress
Volume8
Issue6
Start Page572
Pagination572 - 575
Date Published11/1992
Abstract

The mitochondria and actin fibers of 3T3 fibroblasts cultured on microcarriers in spinner flasks were visualized using fluorescent stains. In contrast to cells grown on planar surfaces under static or steady laminar flow conditions, cells exposed to higher levels of turbulent agitation do not form actin stress fibers. Greater agitation also leads to a more diffuse appearance of the mitochondria and a wider distribution of them throughout the cytoplasm. This response may indicate damaged mitochondria, as similar results have been reported for chemical toxins.

DOI10.1021/bp00018a600
Short TitleBiotechnology Progress